解剖学报

›› 2012, Vol. 43 ›› Issue (1): 68-71.doi: 10.3969/j.issn.0529-1356.2012.01.013

• 肿瘤生物学 • 上一篇    下一篇

宫颈鳞癌患者血清膜联蛋白A5含量的检测及其临床意义

李欣1*; 王立平2; 梁秀军1; 邢恩鸿2; 高玉峰1   

  1. 1.承德医学院基础医学研究所,河北 承德 067000; 2. 承德医学院附属医院中心实验室, 河北 承德067000
  • 收稿日期:2011-09-05 修回日期:2011-10-19 出版日期:2012-02-06
  • 通讯作者: 李欣

Clinical significance of serum annexin A5 in patients with uterine cervical squamous cell carcinoma

  1. (1. Basic Medcine Institute, Chengde Medical College，Hebei Chengde 067000, China; 2. Central Laboratory, the Affiliated Hospital of Chengde Medical College，Hebei Chengde 067000, China
  • Received:2011-09-05 Revised:2011-10-19 Online:2012-02-06
  • Contact: LI Xin

关键词: 膜联蛋白A5, 外周血, 宫颈鳞癌, 鳞状细胞癌抗原, 酶联免疫吸附试验, 人

Abstract: Objective To investigate whether ANXA5 could be used as a diagnose marker for human uterine cervical squamous cell carcinoma. Methods The blood of 38 normal women and 48 uterine cervical squamous cell carcinoma patients was collected. The serum and red blood cells were obtained by low speed centrifugation. The content of ANXA5 and SCCAg was detected by ELISA method and RT-PCR was used to determine the mRNA expression of ANXA5 The data of the two groups were analyzed by t test and the relativity analysis was done between ANXA5 and SCCAg. Results According to ELISA method, the contents of both ANXA5 and SCCAg in the serum of uterine cervical carcinoma patients were higher than that of normal women（EM>P /EM><0.05). RT-PCR results showed that ANXA5 mRNA level in the patients was higher than that of normal（EM>P/EM> <0.05）. The relativity analysis showed that ANXA5 and SCCAg had a positive correlation, but the positive ratio of ANXA5 was higher than that of SCCAg. BR>Conclusion The content of ANXA5 in the uterine cervical carcinoma patients increased significantly with high speciality and sensitivity and might be used as a

Key words: Annexin A5, Circulated blood, Uterine cervical squamous cell carcinoma, Squamous cell carcinoma antigen, ELISA, Human

中图分类号: